Sucrose gradient centrifugation sedimentation standards: a simplified technique.
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چکیده
منابع مشابه
A method for determining the sedimentation behavior of enzymes: application to protein mixtures.
During an investigation of the gene-enzyme relationships in histidine biosynthesis in Xalmonella typhimurium, it became desirable to determine, in crude extracts, the approximate molecular weights of several enzymes. We have found sucrose gradient centrifugation to be a suitable method for determining sedimentation coefficients of enzymes in protein mixtures. A variety of enzymes of known prope...
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Because of their intrinsic low buoyant density, chitosomes can be separated from crude cell homogenates (1000 g or 35,000 g supernatants) of Mucor rouxii by isopycnic sedimentation in sucrose density gradients. To accelerate and simplify the isolation of chitosomes, we centrifuged the cell-free extracts at ultrahigh speed (in a fixed-angle rotor at forces up to 311,000 g Rav) and found that the...
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عنوان ژورنال:
- Clinical chemistry
دوره 26 8 شماره
صفحات -
تاریخ انتشار 1980